A versatile denaturing HPLC approach for human β-globin gene mutation screening

β-thalassemia for translating human β-globin gene and RNA interference of α-globin gene expression by lentivirus vectors

Objective: According to the characteristics of α/β-globin gene imbalance caused by α-globin gene accumulation in β-thalassemia, we designed two lentiviral vectors, that is, while we used the lentiviral to mediate full length human β-globin gene, we introduced the interference fragment of lentiviral α-globin gene to adjust the α/β chain ratio by increasing the expression of β-globin and decreasi...

transfection of human hematopoietic stem cells by a gene targeting construct containing the β-globin gene

background: replacment of cd133+ cell’s β globin gene by using a gene targeting construct containing the β globin gene and essential elements for homologous recombination. materials and methods: pfbggt was amplified, then digested using the nhei and xhoi restriction enzymes, and finally, a 13.3 kb band (naked dna) was extracted from the agarose gel. biological activity of positive and negative ...

construction and evaluation of a specific nonviral vector for ex-vivo gene correction of human β-globin gene defects

Denaturing HPLC procedure for factor IX gene scanning.

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Quantitative mitochondrial DNA mutation analysis by denaturing HPLC.

BACKGROUND In recent years, denaturing HPLC (DHPLC) has been widely used to screen the whole mitochondrial genome or specific regions of the genome for DNA mutations. The quantification and mathematical modeling of DHPLC results is, however, underexplored. METHODS We generated site-directed mutants containing some common mutations in the mitochondrial DNA (mtDNA) tRNA(leu) region with differe...